ВЛИЯНИЕ СТИМУЛЯЦИИ ЦЕНТРАЛЬНЫХ ХОЛИНЕРГИЧЕСКИХ И НОРАДРЕНЕРГИЧЕСКИХ МЕДИАТОРНЫХ СИСТЕМ И ПЕРИФЕРИЧЕСКИХ ВЕГЕТАТИВНЫХ БЛОКАД НА ВОЛНОВЫЕ ХАРАКТЕРИСТИКИ ВАРИАБЕЛЬНОСТИ СЕРДЕЧНОГО РИТМА

nervous tissue or in the incubation medium in the presence of L-leucine 
should be considered as a direct evidence of DA synthesis by 
monoenzymatic TH neurons in cooperation with any other neurons, 
possessing AADC.

The L-DOPA concentration in incubation medium was 0,027 ± 0,003 

ng/ml. It was maintained at the same level after incubation in KrebsRinger solution with L-leucine (0,033 ± 0,003 ng/ml). However according 
to our hypothesis incubation with leucine should not only decrease DA 
levels but also increase L-DOPA levels in the incubation medium. This 
inconsistency could be
explaned this way: both L-DOPA and DA are 

metabolized by COMT, therefore when DA levels decrease this could 
attenuate L-DOPA affinity to COMT, increasing its degradation via this 
enzyme. According to this assumption COMT inhibition should cause LDOPA increase in incubation medium with L-leucine in comparison with 
the medium without L-leucine. In order to test this hypothesis, we have 
performed a second series of experiments by incubating the MBH 
initially in Krebs-Ringer solution with tolcapone, an inhibitor of 
COMT, and then in the same solution containing or lacking L-leucine. SN 
was used as a control. The L-DOPA concentration in incubation medium of 
the Krebs-Ringer solution, containing tolcapone and L-leucine was 
significantly higher than in Krebs-Ringer solution containing only 
tolcapone - 0,14 ± 0,04 ng/ml vs. 0,23 ± 0,03 ng/ml.

Thus, the inhibition of the cooperative synthesis with L-leucine 

perfusion decreases DA levels by 40% both in tissue and in medium and 
increases L-DOPA levels by 39% in the medium, that conclude the 
significant role monoenzymatic neurons in the DA synthesis in MBH of 
adult rats. 
References

1. Ershov P.V., Ugrumov M.V., Calas A., Krieger M., Thibault J. //J. 

Comp. neurol. 2002. V. 446. № 2. P. 114-122.

2. Misu Y., Kitahama K., Goshima Y. //Pharmacol. Therapeutics. 2003. 

V. 97. P. 117-137. 

3. Ugrumov M.V.
Journal of Chemical Neuroanatomy. 2009. V.
38. P. 

241–256. 

4. Ugrumov 
M., 
Melnikova 
V., 
Ershov 
P. 
et 
al. 

//Psychoneuroendocrinology. 2002. V. 7. P. 533-548.  

DOI:10.12737/12401

ВЛИЯНИЕ СТИМУЛЯЦИИ ЦЕНТРАЛЬНЫХ ХОЛИНЕРГИЧЕСКИХ И 

НОРАДРЕНЕРГИЧЕСКИХ МЕДИАТОРНЫХ СИСТЕМ И ПЕРИФЕРИЧЕСКИХ 

ВЕГЕТАТИВНЫХ БЛОКАД НА ВОЛНОВЫЕ ХАРАКТЕРИСТИКИ ВАРИАБЕЛЬНОСТИ 

СЕРДЕЧНОГО РИТМА

Курьянова Е.В., Жукова Ю.Д., Теплый Д.Л.

Астраханский государственный университет, кафедра физиологии, 

морфологии, генетики и биомедицины

fyzevk@rambler.ru