СЕКРЕТОРНАЯ АКТИВНОСТЬ ОРГАНОВ СИНТЕЗИРУЮЩИХ НОРАДРЕНАЛИН В ОНТОГЕНЕЗЕ У КРЫС

the effect of melatonin in different concentrations on synaptic 
transmission in system of Shaffer collaterals – pyramidal neurons of 
CA1 area of hippocampus, as well as the ability of melatonin to induce 
epileptiform activity. 
METHODS
The experiments were performed in hippocampal slices of 15 non-pedigree 
albino male rats weighing 200–220 g. Preparation, incubation of slices 
and composition of artificial cerebrospinal fluid was described by us 
earlier [2]. Stimulation of Schaffer collaterals was performed with a 
frequency of 1 Hz (30 pulses for 30 sec), registering all population 
spikes (PS). Statistical processing of the research results was 
performed using the software BioStat 2009 Professional. To assess the 
significance of differences of two samples was used t-Student test.
RESULTS
It was found that perfusion of rat hippocampal slices by artificial 
cerebrospinal fluid with melatonin in concentration of 0,5 mM (n=6) 
didn’t change orthodromic PS considerably: its latent period, amplitude 
and form almost didn’t change. 
Melatonin in concentration 2 mM significantly (p<0,05)
depressed 

orthodromic PS on 243% (n=7), in concentration 5 mM
– on 726% (n=8; 

p<0,01) and didn’t cause epileptiform activity.
Thus, melatonin depressed synaptic transmission in system of Shaffer 
collaterals – pyramidal neurons of CA1 area of hippocampus and didn’t 
cause epileptiform activity. The increase of melatonin concentration 
(from 0,5 to 5 mM) increases its effect.

REFERENCES

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Arushanian E.B.  // Eksp Klin Farmakol. 2015. Vol. 78. No 3. P. 40–

43.
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Motin V.G. // Bull Exp Biol Med. 2011. Vol. 152. No 7. P. 32–36.

3.
Coras R., Pauli E., Li J. et al. // Brain. 2014. Vol. 137, Pt. 7. 

P. 1945–1957.
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Musshoff U., Riewenherm D., Berger E. et al. // Hippocampus. 2002. 

Vol. 12. No 2. P. 165–173.
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Wang L.M., Suthana N.A., Chaudhury D. et al. // Eur. J. Neurosci. 

2005. V. 22. No 9. P. 2231–2237.
DOI:10.12737/12427

СЕКРЕТОРНАЯ АКТИВНОСТЬ ОРГАНОВ СИНТЕЗИРУЮЩИХ НОРАДРЕНАЛИН В 

ОНТОГЕНЕЗЕ У КРЫС

А.Р. Муртазина, Н.С. Бондаренко, Ю.О. Зубова, А.Я. Сапронова,

М.В. Угрюмов

Федеральное государственное бюджетное учреждение науки

Институт биологии развития им. Н.К. Кольцова РАН
Научный руководитель – академик РАН М.В. Угрюмов

Aliya_mr89@mail.ru

Работа 
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активности 
органов 

синтезирующих норадреналин в онтогенезе у крыс В ходе выполнения данной