ЭФФЕКТЫ ПРОЛИН-БОГАТОГО ПЕПТИДА СИСТЕМЫ ВРОЖДЕННОГО ИММУНИТЕТА – CHBAC3.4 НА ОПУХОЛЕВЫЕ КЛЕТКИ ЧЕЛОВЕКА

executed on device BioSpec 70/30 (“Bruker”, Germany) with an induction 
of a magnetic field 7 Tl and gradiente system of 105 ¼Ô½/m. The 
morphometric analysis of MRT-images was spent in program ImageJ 1.38. 
(National Institutes of Health, USA). The severity of TBI was estimated 
on the serial stained histologic sections by light microscopy. Data 
were analyzed statistically using the computer program Statistica 6.0 
(Statsoft, USA). Intragroup comparisons were made using the MannWhitney U test. Statistical differences in infarct volume was accessed 
using Student's t-test.
Results of research.
Before operation all experimental animals have 

typed 14 points in the “Limb placing test”. Animals of the first 
(control) group have repeated the test for 9-day for 14 points. The 
animals of the 2nd group have typed 11,6±0,3 points (P<0,05), the 3rd

group –
10,45±0,3 points (Р<0,05); the 4th
group –
9,1±0,4 points 

(Р<0,01). A histologic study of the brain slices revealed, that the 
properties of necrotic areas in the center of traumatic damage depends 
on the height from which the weight was falled. At the height of 10 sm, 
the depth of necrotic damage was limited by IV-V (internal granular and 
ganglionary) layers, and in subcortical structures, first of all in 
hippocampus, a perivascular oedema was observed. At the height of 15 
sm, a necrosis on all cortical depth was defined, and in hippocampus 
and striatum f perivascular and  pericellular oedema was revealed. At 
the height of 20 sm, besides the deep necrosis in damaged hemisphere 
the displacement of subcortical structures caused by a significant 
brain oedema was marked. It should be noted the appearance of exofocal 
sites with the damaged ischemic neurons in the contralateral (intact) 
hemisphere formed, probably,
owing to posttraumatic oedema of brain 

matter. MRT-research of a brain showed that the average volume of 
damage also depends on the height from which the weight was falled. At 
the height of 15 cm the volume was 81,5±3,4 mm3, and at the height of 10 
cm it was 35,83±2,3 mm3.
Thus, functional disturbances were confirmed by MRT as well as 
morphological 
and 
histologic 
studies. 
The 
comparison 
of 
these 

parameters will contribute to  prognosis of rehabilitation potential at 
different severity of TBI in experiment.
References:
1.Feeney D.M. et al. Responses to cortical  injury: Methodology and  
local effects of contusions in the rat.//  Brain Res.1981; 211: 67-77.
2.Paxinos G., Watson C. Atlas of anatomy of rat brain// In: The Rat 
Brain in Stereotaxic Coordinates. 3nd San Diego, Calif. Academic Press 
Inc; 1997.
DOI:10.12737/12487

ЭФФЕКТЫ ПРОЛИН-БОГАТОГО ПЕПТИДА СИСТЕМЫ ВРОЖДЕННОГО ИММУНИТЕТА

– CHBAC3.4 НА ОПУХОЛЕВЫЕ КЛЕТКИ ЧЕЛОВЕКА

Шамова О.В.*, **,, Пазина T.Ю.*, Жаркова М.С.*, Артамонов А.Ю.*, Юхнев

В.А.*, Гринчук Т.М.***, Орлов Д.С.*,
**