ПРОЛИФЕРАЦИЯ КЛЕТОК В МОЗГЕ МЫШЕЙ ПРИ ФОРМИРОВАНИИ ОБСТАНОВОЧНОГО УСЛОВНО-РЕФЛЕКТОРНОГО СТРАХА

significantly increased (1.12 [0.64; 1.21] ng / ml and 9.5 [2.0, 9.9] m 
/ ng, p <0,01 in both cases). The increasing concentration of rDNA 
directly correlated with increased PA (r = 0,68, p = 0,02), which was 
52 ± 5%. In the blood plasma of patients with the II-nd degree EH, 
cfDNA concentration was much higher than the normal values (290 [179; 
890] ng / ml, p <0,05 p <0,01), correlating with an increase PA (r = 
0,62, p = 0.004) and combining with an increase in DR (P <0,001).

Addition of cfDNA samples 1-5 ng /ml from the control donors into 

the culture of endothelial cells stimulated 4-fold increase in the NO 
concentration in both cells and extracellular medium, but addition of 
cfDNA samples from hypertensive patients, conversely, reduces it to 
1,3-28 times. There was an inverse exponential relationship between the 
rDNA content in the pool of cfDNA and NO concentration  in the 
endothelial cells culture (linear regression coefficient is k = - 0,98, 
p <0,0001).

Thus, CpG-rich genome sequences accumulating in the pool of cfDNA, 

participate in the system mechanisms of stress reactions that lead to 
the development of EH. Normally, cfDNA promotes to improving blood 
supply to organs and maintaining normal blood pressure, by improving 
the blood flow, on the one hand, and by stimulation of endothelial 
receptors 
and 
NO-mediated 
vasorelaxation, 
on 
the 
other 
hand. 

Significant accumulation of CpG-rich genome repeats in the pool of 
cfDNA inhibits synthesis of NO by endothelial cells, promoting 
vasoconstriction and deficiency of NO, possibly being the cause of 
endothelial dysfunction at the beginning of EH, on the one hand, and 
increases the PA, inhibiting blood circulation and contributing to a 
greater increase in blood pressure, on the other hand.

LITERATURE

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Veiko N.N. // Bull Exp Biol Med. 2012. Vol. 153, 

No 3. P. 305-308.

2. Hanke M.L., Kielian T. // Clin. Sci (Lond). 2011. Vol. 121. P. 367
387.

3. Kumagai Y, Takeuchi O, Akira S. // Adv. Drug Deliv. Rev. 2008. Vol. 

60. P. 795–804.

4. Montecucco F., Pende A., Quercioli A., Mach F. // JNEPHROL. 2011. 

Vol. 24, No 01. P. 23- 34.

5. Veiko N.N., Konorova I.L., Neverova M.E. et al. // Biochemistry 

(Moscow) Supplement Series B: Biomedical Chemistry. 2010. Vol. 4, 
No. 3. P. 269-278. 

DOI:10.12737/12385

ПРОЛИФЕРАЦИЯ КЛЕТОК В МОЗГЕ МЫШЕЙ ПРИ ФОРМИРОВАНИИ 

ОБСТАНОВОЧНОГО УСЛОВНО-РЕФЛЕКТОРНОГО СТРАХА

Копаева М.Ю.*, Ефимова О.И.*, Анохин К.В.*,**

*НИЦ «Курчатовский институт», **ФГБНУ «НИИНФ им. П.К. Анохина», Москва, 

Россия

efimova_oi@nrcki.ru