ФИЗИОЛОГИЧЕСКИЕ ОСОБЕННОСТИ КООРДИНАЦИИ ДВИЖЕНИЙ В УСЛОВИЯХ ЧАСТИЧНОЙ СЕНСОРНОЙ ДЕПРИВАЦИИ

Kamyshnyi A. M., Topol I. A.

Zaporozhye State Medical University

alexkamyshny@yandex.ru

Abstract. Stress-induced immune disregulation is a risk factor of 

autoimmune and inflammatory diseases, but to date, the mechanisms for 
this effect are not fully known.

Key words: chronic social stress, GALT, Nr3c1, Adrb2, IL-1β, IL
17а, Nlrp3.

It is investigated the mRNA expression levels of NR3C1,
Adrβ2,

pro-inflammatory
cytokines
IL-1β, IL-17а and Nlrp3 inflammasome
in 

gut-associated lymphoid tissue (GALT) under CSS in Wistar rats. There 
were revealed the peculiarities of the distribution RORγt+-, Foxp3+-, 
LMP2+-, XBP1+ lymphocytes in GALT rats in conditions of CSS.

Experiments were carried out on female Wistar rats aged 5–6 

months. To determine the level of mRNA expression target genes
was 

performed RT-PCR in real-time by
thermocycler CFX96™ Real-Time PCR 

Detection Systems («Bio-RadLaboratories, Inc», USA). The relative level 
of gene expression were studied with rat reference genes GAPDH by the 
method 
ΔΔCt. Statistical analysis were conducted using available 

software «Bio-Rad СFX Manager 3.1» (Bio-Rad, USA). The immunopositive 
lymphocytes were determined using an indirect immunofluorescence 
technique with using a monoclonal rat antibody.

CSS development leads to decrease the mRNA expression levels of 

Nr3c1 and Adrβ2-receptors in GALT cells and is accompanied by 
unidirectional dynamic to increase the transcriptional activity of the 
genes 
of 
pro-inflammatory 
cytokines 
IL-1β, 
IL-17a 
and 
Nlrp3
inflammasome. These changes are accompanied by a decrease in the ratio
of Foxp3+/Roryt+ cells, and the predominance of Th17-differentiation on 
the background of suppressive failure. In addition, CSS development was 
accompanied by a unidirectional tendency to increase the total number 
of LMP2+ lymphocytes and reduction of population density ХВР1+ cells in 
lymphoid structures of rat ileum.

Events occurring at the GALT under CSS contradict the classical 

paradigm of stress. These processes are not provoking immunosuppressive
effects, but demonstrate activation of the immune system and the 
inflammatory process.
DOI:10.12737/12363

ФИЗИОЛОГИЧЕСКИЕ ОСОБЕННОСТИ КООРДИНАЦИИ ДВИЖЕНИЙ В УСЛОВИЯХ 

ЧАСТИЧНОЙ СЕНСОРНОЙ ДЕПРИВАЦИИ

Л.В. Капилевич1,2, О.С.Смердова2 , А.В.Разуванова2 , Е.В.Кошельская2,3

Томский государственный университет1, Томский политехнический 

университет2, Томский областной институт повышения квалификации 

работников образования3 , Томск, Россия

kapil@yandex.ru