АНАЛИЗ АДАПТИВНОСТИ ЧЕЛОВЕКА К УСЛОВИЯМ НОВИЗНЫ ДЕЯТЕЛЬНОСТИ ПО ПОКАЗАТЕЛЯМ РЕЗУЛЬТАТИВНОСТИ И ВАРИАБЕЛЬНОСТИ КАРДИОРИТМА ПРИ СЕНСО-МОТОРНОМ ТЕСТИРОВАНИИ

that 50% of neurotransmitter quanta were released within 0.88±0.05 msec 
and 90% within
1.19±0.07 msec. Denervation (seven experiments, 

extracellular Ca2+
ion concentration 0.25-0.3 mM, the EPC quantal 

content was essentially identical to control experiments) lead to an 
increase in desynchronization of quantal release. The distribution of 
synaptic delays had a mean of 1.85±0.19 msec, a dispersion of 
1.92±0.27; construction of cumulative curves demonstrated that 50% of 
neurotransmitter quanta were released within 1.06±0.03 msec and 90% 
within 3.52±0.47 msec. The topography of transmitter release in nerve 
endings was studied using a three-microelectrode method [5]. In control 
transmitter release did not occur throughout the whole surface of the 
nerve ending, but only in specific sites. The configurations and sizes 
of the transmitter release sites, as well as the distances between 
them, were identical to the positions and sizes of active zones 
described in ultrastructural studies [3, 5, 1]. Denervation caused 
groupings of release sites lost their outlines, became blurred, and 
transmitter release occurred over the whole surface of the nerve 
ending. But in some experiments increased probability of transmitter 
release in certain synaptic regions were observed that likely coincided 
with the location of large fragments of partially disorganized active 
zones. 

Thus, obtained data allowed to consider that denervation caused 

disorganization of active zones that led to decrease in synatic 
efficiency and increase in the time course of transmitter release. The 
results presented here are in agreement with previously obtained data 
on the studying of transmitter release in the frog neuromuscular 
junction after exposure to calcium-free solution that was another 
experimental approach led to disorganization of active zones probably 
due to impairment of the cytoskeleton [1]. The work was supported by 
RFBR (grant № 14-04-01232-а) and RSСF (grant № 14-15-00847).

References.
1. Zefirov A.L., R. D. Mukhamedzianov,M. G. Minlebaev et al. // 

Ross Fiziol Zh Im I M Sechenova. 2002. V.88, №2. P. 191-204. 
2. Ko C.P. // J. Physiol. 1981. Vol. 321. P. 627-639.
3. Pawson P.A., Grinnel A.D., Wolowske B. // 1998. J. Neurocytol. Vol. 
27. P. 379-391.

4. Rizzoli S.O. // The EMBO Journal. 2014. Vol. 33, №8. P. 788
822.
5. Zefirov A.L., Benish T., Fatkullin N., et. al. // Nature. 1995. Vol, 
376. P. 393-394. 

DOI:10.12737/12328

АНАЛИЗ АДАПТИВНОСТИ ЧЕЛОВЕКА К УСЛОВИЯМ НОВИЗНЫ ДЕЯТЕЛЬНОСТИ 
ПО ПОКАЗАТЕЛЯМ РЕЗУЛЬТАТИВНОСТИ И ВАРИАБЕЛЬНОСТИ КАРДИОРИТМА 

ПРИ СЕНСО-МОТОРНОМ ТЕСТИРОВАНИИ

Гриднева Н.А., Муртазина Е.П., Журавлев Б.В.

ФГБНУ НИИ нормальной физиологии им.П.К.Анохина, Москва, РФ

n.gridneva@nphys.ru