СОЗДАНИЕ ХРОНИЧЕСКОЙ МОДЕЛИ РАННЕЙ СИМПТОМНОЙ СТАДИИ ПАРКИНСОНИЗМА У МЫШЕЙ

centers  with reduced macrophage activity in them. There were also 
lymphoid follicles without germinal centers which made 20% of the 
number of total follicles in the spleen. It should be noted that such 
follicles became smaller and irregularly shaped. The red pulp had 
marked regions of extramedullar hemapoiesis in isolated pockets. Thus, 
in this group spleen tissue of experimental animals demonstrated 
reduction of local immune response and appearance of hemapoiesis in 
extramedullar  foci which demonstrated compensatory response to the 
violation of hemapoiesis in bone marrow.

Under the MT + PC radiation morphological indices revealed deeper 

oppression of the local immune homeostasis. In the white pulp of the 
spleen there were dramatically increased degradation and reduction in  
the amount of white pulp. However, only 80% of lymphoid follicles had 
germinal centers which have been drastically reduced and deformed. 
Periarterial lymphoid coupling also decreases sharply, exposing the 
pulp artery. The walls of some arteries were thickened by hyalinosis up 
to obliteration of the lumen. Only 20% of lymphoid follicles had 
narrowed germinal centers with separate macrophages. The proliferative 
activity of lymphoid cells decreased. In periarterial lymph sheaths 
there was detected sharp narrowing of the lumen and the reduction of 
cellular elements in them,
as the number of lymphoid cells and 

macrophages. The red pulp cords had marked thickening of the pulp and 
connective trabeculae, the emergence of multiple foci of extramedullar 
hemapoiesis, 
impaired 
circulation 
to 
the 
formation 
of 
small 

hemorrhages.

Thus,
the effect of electromagnetic radiation caused by the 

combined use of a PC and the MT leads to structural changes of the 
spleen, indicating a significant decrease of the local cellular and 
humoral immune homeostasis. Fibrosis of spleen red pulp trabeculae and 
vessels leads to increased ischemia and impaired hemapoiesis. Less 
pronounced inhibition of white pulp lymphoid tissue of the spleen of 
rats was observed in the group of animals with either MT or PC 
radiation whereby compensatory response was considered as extension of 
the germinal centers of lymphoid follicles which imitated immune 
response to the antigen.

Refrences:

1. Yaschenko S.G., Rybalko S.J., Kolbasin P.N. // Proceedings of the 
Southern Branch of the National University of Life and Environmental 
Sciences of Ukraine. 2013. N155. Р. 305-309. [In Russian]
2. Shapkin U.G., Maslaykov V.V. // Herald of surgery. 2009. Vol. 168, 
N2. P.110-113. [In Russian]
3.  Mebius R. E., Kraal G. // Nat. Rev. Immunol. 2005. N 5. P. 606–616.
DOI:10.12737/12455

СОЗДАНИЕ ХРОНИЧЕСКОЙ МОДЕЛИ РАННЕЙ СИМПТОМНОЙ СТАДИИ 

ПАРКИНСОНИЗМА У МЫШЕЙ

В.В. Сафандеев1, А.А. Колачева1, А.Р. Ким1, Е.А. Козина1, М.В. Угрюмов1.