РЕГУЛЯЦИЯ АНГИОГЕНЕЗА В ЗОНЕ ЭКСПЕРИМЕНТАЛЬНОГО ИНФАРКТА МИОКАРДА ПУТЕМ ПРИМЕНЕНИЯ МУЛЬТИПОТЕНТНЫХ МЕЗЕНХИМАЛЬНЫХ СТВОЛОВЫХ КЛЕТОК В ЭКСПЕРИМЕНТЕ

criteria for inclusion in the control group were: age 40-85 years, 
normal blood pressure, BMI within 18,5-25,0.
A survey was carried out with all research participants . The 
questionnaire 
presented 
questions 
on 
risk 
factors. 
After 
the 

questioning, all participants underwent the following survey methods: 
measurement of BMI, SBP, DBP, respiratory rate, heart rate, determining 
the 
level 
of 
glucose, 
glycosylated 
hemoglobin 
determination, 

investigation of blood coagulation  (PTI, the amount of fibrinogen, 
platelet 
aggregation, 
aPTT), 
biochemistry 
parameters 
of 
blood  

(cholesterol, 
triglycerides, 
ALT, 
AST, 
total 
bilirubin, 
direct 

bilirubin, urea, creatinine, total protein), ECG and Doppler sonography 
of the brachiocephalic trunk. Determination of the markers NSE, GFAP, 
MMP-9 was conducted at the Laboratory of Collective Use of the 
Karaganda State Medical University. The lysed samples were excluded 
from the study. To determine the markers, the following sets of 
reagents were used: Human GFAP ELISA (BioVendor), Human MMP-9 ELISA 
(Bender MedSystems). Enzyme immunoassay was performed on the robot 
Tecan Evolizer 100. The results were evaluated: for NSE in mg / l, for 
GFAP and MMP-9 ng / ml. Statistical processing of the obtained data 
included determination of the correlation coefficients and the 
development of a logistic regression model, describing the dependence 
of stroke and 35 studied parameters was developed.
Results. The obtained mathematical model allows: to determine the risk 
of stroke in a patient or a tendency of stroke rise in social groups,
to explore the character of change in the probability of occurrence of 
stroke according to operating factors, to assess the degree of 
influence of the studied factors on the probability, to predict the 
occurrence of stroke for given levels of the factors, to determine the 
optimal levels of factors to indicate required values of parameters. 
Processing of the results of laboratory studies with markers would 
change predictive model.
Conclusion. The development of predictive technique «InRisk» and the 
study of
markers NSE, GFAP and MMP-9 allowed to implement
early 

diagnostics of asymptomatic brain damage in patients with the metabolic 
syndrome. This prevents them from developing acute stroke.
REFERENCE:
1. Методика прогнозирования вероятности возникновения инсульта., Г. Т. 
ЕсенбаеваС. Б. Жаутикова  Ф. А. Миндубаева  И. А. Кадырова, Журнал 
неврологии и психиатрии им. С.С. Корсакова
2014; 3: 51-54

2. 
Microstructural 
Brain 
Tissue 
Damage 
in 
Metabolic 
Syndrome 

MichielSala, Albert de Roos, Annette van den Berg1, Irmhild AltmannSchneider1, P. ElineSlagboom, Rudi G. WestendorpDiabetes Care October 2, 
2013, doi: 10.2337/dc13-1160
DOI:10.12737/12421

РЕГУЛЯЦИЯ АНГИОГЕНЕЗА В ЗОНЕ ЭКСПЕРИМЕНТАЛЬНОГО ИНФАРКТА 
МИОКАРДА ПУТЕМ ПРИМЕНЕНИЯ МУЛЬТИПОТЕНТНЫХ МЕЗЕНХИМАЛЬНЫХ 

СТВОЛОВЫХ КЛЕТОК В ЭКСПЕРИМЕНТЕ

1В. Ю. Михайличенко, 1С.А. Самарин.