ROLE OF PRO-INFLAMMATORY S100 PROTEINS IN AMYLOID DISEASES

dopamine receptors (D1, 1:200) were used. The optical density (OD, in 
arbitrary units)  of THf40 and THf31 was determined  in  images of 
dopaminergic neurons of the substantia nigra (SN) and the ventral 
tegmental area (VTA).  Statistical analysis was performed using t-test. 
Results are presented as mean ± standard error and in percents. The 
double 
immunohistochemical reaction was performed on midbrain area 

sections.  The primary rabbit antibodies to the phosphorylated protein 
kinase-A (fPKA, Abcam, UK; 1: 100) and mouse to the TH (Sigma, USA, 1: 
1000) were used as previously described [3] and studied by confocal 
microscopy (Leika XP5).
RESULTS
In Ay /a mice in obesity and decrease the level of AgRP in brain 
dopaminergic neurons in the arcuate nucleus, zona incerta, substantia 
nigra, ventral tegmental area the OD of  THf40 was increased on 25-30% 
compared to a/a mice. Significant differences of  THf31 OD in 
dopaminergic neurons and in areas of dopamine innervation was not 
detected. 
The 
double 
immunolabeling 
indicated 
 
that 
the 

immunoreactivity of  fPKA in dopaminergic neurons in the midbrain 
substantia nigra and ventral tegmental area was increased. In the 
paraventricular nucleus of the hypothalamus, which is well known, 
receives abundant dopaminergic innervation, the immunoreactivity of 
THf40 and D1   have been increase. The data indicate that the 
activation of dopamine biosynthesis systems in obese Ay/a mice occurs 
during G-protein coupled activation interacting messengers. It is 
suggested that the protein agouti, intracellular overexpressed  in Ay/a 
mice, may have an inhibitory effect on ERK-module and associated 
mechanisms.
Research was  supported by RFBR grant №14-04-31565_ mol-a.
LITERATURE
[1] Bagnol  D., Lu X.Y., Kaelin C.B., Day H.E., Ollmann  M., Gantz  I., 
Akil  H., Barsh G.S., Watson  S.J. Anatomy of an Endogenous Antagonist: 
Relationship between Agouti-Related Protein and Proopiomelanocortin in 
Brain.  J. Neurosci. 1999. V. 19.  P. 1-7. 
[2] Makarova, E.N., Yakovleva, T.V., Shevchenko, A.Y. Pregnancy and 
lactation have antiobesity and anti-diabetic effects in Ay/a mice // 
Acta Physiologica.  2010.  V. 198 (2). P. 169-177. 
[3] Mikhrina A. L., Romanova
I. V.  //Neurosci. Behav. Physiology. 

2015, V. 45 (5).  P. 536-541.
[4] Ollmann M.M., Wilson  B.D., Yang  Y.K., Kerns  J.A., Chen Y., Gantz 
I., Barsh  G.S..// Science.  1997. V. 278. P. 135-138. 
[5] Romanova I. V., Mikhrina  A. L.  //Human Physiology.  2013.  V. 39 
(6).
P. 584-589. 

DOI:10.12737/12425

ROLE OF PRO-INFLAMMATORY S100 PROTEINS IN AMYLOID 

DISEASES

Ludmilla A. Morozova-Roche

Department of Medical Biochemistry and Biophysics, Umeå University, 

Umeå, Sweden